Traditional Chinese Medicine (TCM) has been widely and successfully used in treating illnesses ranging from inflammation to cancer, and compounds from medicinal herbs and minerals are playing more and more important roles in taming various kinds of diseases
Xi’an Hao-xuan Bio research and extract the high purity Oridonin 98% HPLC from the RR, supplied to the both medical institution for research and pharmaceutical enterprise. We investigated the effects of oridonin on a spectrum of leukemic cells including the t(8;21)-harboring Kasumi-1 cell line and primary leukemia cells isolated from patients with de novo or relapsed/refractory t(8;21) AML. In our work, cell proliferation, cell viability, cell cycle, mitochondrial transmembrane potential, externalization of phosphatidylserine, and in situ cell death were carefully analyzed. We found that among cells tested, t(8;21)-harboring cells were the most sensitive ones to oridonin treatment . Oridonin at clinical available concentrations inhibited growth and induced programmed cell death of t(8;21) leukemic cells through insult of mitochondria functions and unleash of apoptosis machineries, down-regulation of apoptosis antagonist Bcl-2, and activation of apoptosis executioners including caspase-9 and -3. Since AE fusion protein is critical to leukemogenesis, we assessed the impact of oridonin on AE and found that oridonin treatment could cause a caspase-3 dependent degradation of AE, and the aspartic acid residue at position 188 of AE (D188) was identified as the cleavage site.